A chromosome-level genome assembly of the beavertail cactus, Opuntia basilaris

Few genomic resources currently exist for the American endemic family Cactaceae, a group of around 1850 species, which are world-renowned for their amazing growth forms and succulent habits. These icons of arid landscapes across the Americas are threatened in many parts of their range, including in parts of California, and developing more comprehensive genomic data will aid efforts to better understand and preserve these plants. We sequenced and assembled the genome of the beavertail cactus, Opuntia basilaris, which is represented by three varieties in California, one of which is threatened, and another endangered. The genome assembly has a BUSCO complete score of 98.1%, and a total scaffold length of 980 Mb, with a scaffold N50 length of 83 Mb. The genome size of diploid O. basilaris is markedly smaller than other diploid members of Cactaceae that have been assembled to date. This is the first nuclear genome sequenced in subfamily Opuntioideae and the most complete nuclear genome ..., , # A Chromosome-level Genome Assembly of the Beavertail Cactus, *Opuntia basilaris*

Dataset DOI: [10.5061/dryad.mpg4f4rbp](10.5061/dryad.mpg4f4rbp)

## Description of the data and file structure

Data represent supplemental figures and tables associated with the publication of a chromosome-level assembly genome of *Opuntia basilaris* var. *basilaris* (Cactaceae).

### Files and variables

#### File: SupplementaryFigure1.dcOpuBasi1.hifi.readlength.distribution.png

**Description:** Read length distribution.

#### File: SupplementaryFigure2.IRplusOutput.dcOpuBasi1.chloroplast.pdf

**Description:** Map of inverted repeats in chloroplast genome.

#### File: SupplementaryFigure3.blobtools\_dcOpuBasi1.NCBI.a\_ctg.snail.png

**Description:** Snailplot of alternative genome assembly.

#### File: Supplementary\_Tables.xlsx

**Description:** List and description of removed contaminants.
Tables correspond to Blobtools output per haplotype. The tables show information per contig related to coverage...,