Characterization of nucleolus-associated domains in mus musculus and their dynamics after inactivation of the Upstream binding factor protein

Nucleolus-associated DNA was isolated from MEF cells before and after conditional knock-out of UBF and hybridized against genomic DNA in biological replicates. Two different types of immortalized MEF cells were used. MEFs were immortalized by genetic depletion of p53, iMEFs were immortalized by transfection of the SV40 Tt antigen. Two biological replicates of wild-type vs flox cells of MEF and iMEF cells before and three days after UBF knock-out.