Control of Th17 cell development by Class IIa HDAC4 and HDAC7 through transcriptional repression of negative regulators

We analyzed the genome wide distributions of HDAC1, HDAC4, HDAC7 in Th17 cells. We find that majority of HDAC4 and HDAC7 binding sites are HDAC1 bound. TMP269 inhibits HDAC4 and HDAC7 at promoter sites of Th17 negative regulator genes, leading to their upregulation through increased H3, H4 acetylation. Overall design: ChIP-seq study of HDAC1, HDAC4, and HDAC7 in Th17 cells. The Th17 cells are differentiated for 48hrs and ChIP with the corresponding antibodies and ChIP-seq libraries are made.