Metagenome of nitrifying membrane aerated biofilm before and after temperature change

To improve the design and adaptation of MABR processes for colder climates and varying temperatures, the temperature dependence of a counter-diffusional biofilms nitrification performance was investigated. A lab-scale MABR system with silicone hollow fibre membranes was operated at various temperatures between 8 and 30C, and batch tests were performed to determine the ammonia oxidation kinetics. Biofilm samples were taken at 8 and 24C and analysed with 16srRNA sequencing to monitor changes in the microbial community composition, and a mathematical model was used to study the temperature dependence of mass transfer.