Transcriptome profiling of different celltype of cells (Progenitor cells/Enteroendocrine cells/Enteroblast cells) and Sox100B depleted/overexpressed progenitor cells in Drosophila intestine

Purpose: 1)To compare transcriptome profiling (RNA-seq) of intestinal progenitor cells in Drosophila wild type and Sox100B depleted/overexpressed midgut. 2)To identify cell-type enriched genes and early-transcribed EC/EE genes by comparing transcriptome profiling (RNA-seq) of different celltype of cells Results: Each sequencing experiment generated more than 20 million raw reads, and 92.5% was uniquely mapped for each experiment. 2310 genes of the transcripts showed significantly differential expression between the WT and Sox100B-OE progenitor cells, and 2181 genes of the transcripts showed significantly differential expression between the WT and Sox100B-IR progenitor cells, with a fold change ≥2.0 and p value <0.05. Genes that showed low level in progenitor cells while significantly higher level in terminally differentiated EC/EE cells were identified as early transcribed EC/EE genes. 169 genes were identified as early-transcribed EC genes and 452 genes were identified as early-transcribed EE genes. mRNA profiles of intestinal progenitor cells isolated from 3-day-old wild-type (Ctrl) and Sox100B depleted/overexpressed Drosophila midgut were generated by deep sequencing using Illumina HiSeq 4000.