Histone deacetylase HDA-5 regulates lipid metabolism through H4K5 and H4K8 acetylation in Caenorhabditis elegans

H4K5 and H4K8. The C. elegans N2 were subjected to hda-5 RNAi or empty vectorm. The worm were fed with RNAi strain which target hda-5 until reach to adult. After washing by M9 buffer for three times, worms were re-suspended in PBS, frozen immediately in liquid nitrogen for RNA sequencing which were carried out by Biomarker Technologies (BMK) GmbH (Beijing, China). Total RNA from C. elegans was extracted with RNeasy Plus Mini Kit (Qiagen), assessed for quality by NanoDrop, Qubit 2.0 and Agilent 2100. mRNA was enriched via Oligo(dT) magnetic beads, fragmented, and reverse-transcribed to cDNA with random hexamers. After end repair, A-tailing, adapter ligation and PCR amplification, libraries were qualified by Qubit 2.0, Agilent 2100 and Q-PCR, then sequenced on Illumina NovaSeq 6000 (PE150).