NF-?B encodes history of prior signals to coordinate subsequent cellular responses

We use a novel microfluidic device to provide different concentrations of a point source of TNF-a to a population of NIH3T3 mouse embryonic fibroblasts. These cells are then retrieved in a spatially resolved manner (notated as near, mid, and far from point source) and sequenced using a modified version of the Smart-Seq2 protocol. We find that spatially resolved transcriptomes reveal distinct gene regulation patterns based on TNF dose and/or distance from the point source Overall design: Comparison of triplicate RelA-/- RelA-DsRed NIH3T3 populations exposed to TNF-a at two point concentrations (30 and 100 ng/mL), collected 4 hours after stimulus, and in three positions per sample (near, mid, far).