Gene expression profiles of differentially expressed genes in Enterococcus faecalis 14 ΔBac mutant strain

To explain the particular behavior of the mutant ΔBac strain and the impact of the bacteriocin DD14 on the global regulation and gene expression in Ent. faecalis 14, we performed a comparative transciptomic analysis of RNA isolated from a derivative strain ΔBac mutant versus the wild-type (WT) , after 6 h of growth in GM17 medium under semi-aerobic conditions. Expression data of the 3 wild-type samples were filtered and the average was calculated for each gene. A fold change (FC) value was calculated between ΔBac individual samples and the mean of WT. Differentially expressed genes (DEGs) were selected for a FC threshold > 2.0 or < 0.5.