JBC 2024 - LECT2 Flow Shear

These Figshare files consist of raw/unprocessed images in various formats, processed data and statistical analysis (Graphpad Prism), high resolution versions of some images in the paper, and MATLAB scripts used to analyze/process the microfluidic data. Figure2.zip -Raw light microcsope images of microfluidic aggregates in Zeiss .czi format Figure3.zip -GraphPad Prism files containing processed data derived from images in Figure 2, and statistical analysis Figure4.zip -Raw light microscope images of microfluidic aggregates from seeding experiments, in Zeiss .czi format Figure6.zip -Raw EM images of negatively-stained samples in Gatan .dm3 format -Raw cryo-EM image of frozen vitrified sample in .mrc format -2D average of fibrils -Power spectrum calculated from 2D average Figure7.zip -GraphPad Prism file containing processed data derived from images in Figure 4, and statistical analysis FigureS3.zip -Raw light microscope images of microfluidic aggregates in Zeiss .czi format, from another biological repeat FiguresS5-S11.zip -Raw light microscope images of microfluidic aggregates in Zeiss .czi format -GraphPad Prism files containing processed data derived from above images, and statistical analysis FigureS12.zip -Raw light microscope images of microfluidic aggregates in Zeiss .czi format, testing dependence of aggregation on protein concentration -Graphpad Prism file containing processed data derived from above images, and statistical analysis FigureS14.zip: -Raw light microscope images of microfluidic aggregates from additional biological repeats of seeding experiments, in Zeiss .czi format -GraphPad Prism files containing processed data derived from above images, and statistical analysis ProcessChannelsV6.zip -MATLAB scripts for calculating areas and intensities of aggregates in microfluidic channels