Cross generational effects of a marginal 1-C metabolite deficiency

The effect of a deficiency in one carbon (1-C) metabolites in the adult generation on DNA methylation and gene expression in adult offspring livers was investigated through the use of high-throughput RNA sequencing and reduced representation bisulfite sequencing.Individuals in the F0 generation were either fed a normal diet, or one marginally deficient in 1-C metabolites (folate, vitamins B12 and B6, methionine and choline) from larval stages and mated within each diet group. The resulting offspring were fed the control diet throughout their lives and Livers of adult offspring were harvested after 113 days post fertilisation and RNA and DNA extracted for RNA sequencing and RRBS.