RNA-sequencing (RNAseq) based transcriptomic profiling in primary human valve interstitial cells with depleted PALMD expression

We performed RNA sequencing (RNA-Seq) based transcriptomic profiling in hVICs with depleted PALMD expression by siRNA. Our RNA-Seq analysis revealed 751 altered genes (1.5 fold change, p<0.05), of which 380 genes were significantly down-regulated and 371 genes were significantly up-regulated in hVICs with depleted PALMD.Gene ontology and pathway analysis of the down-regulated genes indicated that PALMD regulated multiple biological processes such as responses to hypoxia and stress, and regulation of cell apoptosis, and several important pathways including glycolysis and TNFα signaling pathway. Gene ontology and pathway analysis showed that the up-regulated genes were associated with the biological processes such as extracellular matrix and structure organization, and multiple pathways including biosynthesis of amino acids, and carbon metabolism. These data suggest that PALMD may be a novel regulator of glycolysis and TNFα signaling in hVICs. Examination of differentially expressed genes in primary human valve interstial cells regulated by PALMD