Nitrogen Input Gradient-Driven Active Ammonia Oxidizers Niche Partitioning Dataset in Protected Vegetable Soils（13CO₂-DNA-SIP）

This dataset encompasses comprehensive information related to ammonia-oxidizing microorganisms in protected vegetable field soils under different nitrogen input gradients, investigated via the 13CO₂-DNA-SIP technique. It includes:
Sample source details: Soil samples from protected vegetable fields subjected to three nitrogen input levels (N0, N522, N870).
Experimental data: Results from 13CO₂-DNA-SIP experiments, covering DNA extraction records, density gradient centrifugation data, and sequencing raw data (in FASTQ format).
Microbial community data: Sequencing analysis outcomes (OTU tables, taxonomic classification results) of ammonia-oxidizing archaea (AOA) and bacteria (AOB), along with their phylogenetic tree files.
Functional and physiological data: Measurements of potential ammonia oxidation rates, gene abundance (amoA gene copies) determined by quantitative PCR, and related environmental factor data (such as soil pH, organic carbon content, and mineral nitrogen concentrations).
The dataset provides a solid foundation for exploring the niche differentiation and ecological responses of active ammonia-oxidizing microorganisms to nitrogen input in intensive protected vegetable production systems.