Transcriptome profile of GEN2.2 cell line after CpG stimulation. Homo sapiens

Analysis of expression profile of human GEN2.2 cell line cultured in the presence of CpG for 2 h. RNA expression profile of the human pDC cell line GEN2.2 after CpG stimulation Overall design: Total RNA was isolated from GEN2.2 cells growing at log phase with High Pure RNA isolation Kit (Roche Diagnostics, Mannheim, Germany), according with the manufacturer´s instructions in triplicate. GEN2.2 cells were stimulated with 1 µM ODN2006 (Life Technologies) for 2h. A total of 300 ng of total RNA was processed using Illumina TotalPrep RNA Amplification Kit. Hybridization of human samples was performed on Illumina Human-HT12 Version 4.