A targeted vector for brain endothelial cell gene delivery and cerebrovascular malformation modelling

In-depth analysis of the molecular mechanisms underlying the initiation and development of bAVM was conducted through single-nucleus RNA sequencing of cells from bAVM lesions in Braf-CAfl/fl mice locally injected with AAV-miniBEND. Overall design: Differential expression analysis and Gene Set Enrichment Analysis of single-nucleus RNA sequencing data. We performed differential expression analysis of RNA seq data and Gene Set Enrichment Analysis obtained from bAVM lesions in Braf-CAfl/fl mice and normal brain region samples from the contralateral hemisphere of the same mice served as a control group.