Single cell mRNA profiling of bone marrow disseminated tumor cells from breast cancer patients

A bone marrow aspirate from a patient with advanced stage breast cancer was subject to size-based disseminated tumor cell (DTC) enrichment using the Parsortix (Angle LLC) 8 micron fluidics device. Cells eluted from the device were used for single cell RNA sequencing to demonstrate the capabilities of the device to enrich for DTCs in an unbiased manner Overall design: A single clinical bone marrow specimen from an advanced stage breast cancer patient was used as a proof of principle