Specificity of rhinocerase towards various fluorescent substrates.
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aThe values are mean ± S.D. (n = 3). 100 µg of rhinocerase were incubated with different concentrations of various fluorescent substrates at 37°C for 7 minutes. The amount of AMC released was measured at an excitation wavelength of 366 nm and an emission wavelength of 460 nm at time intervals up to 7 minutes. 100 µg of B. g. rhinoceros venom were also used at the same experimental conditions for comparison. Kinetic parameters were determined from Lineweaver-Burk plots.
创建时间：
2013-02-21
