These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out . to examine its relation to the hat-like complex.
AD1231: pAD1361/A136; Carbenicillin 50; virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.