Bulk mitotic ATACseq for Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis

We found that Polo-like kinase 1 (Plk1) activity directly on chromatin sustains global transcription and chromosomal accessibility in mitosis. Inhibition of Plk1 leads to significant overall downregulation of accessibility on mitotic chromosomes, especially at promoter and transcription start site (TSS) regions. Therefore, Plk1 activity sustains global chromosomal accessibility in mitosis, facilitating transcription factor binding and ensuring proper transcriptional activity, which maintains mitotic fidelity. Overall design: To study chromosomal accessibility in mitosis and the effect of Plk1 activity, RPE1 Plk1-/- EGFP-Plk1as (Burkard et al., 2007) were arrested in prometaphase before 2 hour DMSO or Plk1 inhibitor 10 µM 3MB-PP1 treatment. Before collection, mitotic index of each sample was measured (>95%). Bulk cell ATAC seq were performed in 4 biological repliactes.