Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature transfer RNA
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https://tandf.figshare.com/articles/dataset/Four_leaf_clover_qRT_PCR_A_convenient_method_for_selective_quantification_of_mature_transfer_RNA/1378876/2
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ABSTRACTTransfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. Here we report the development of Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. In FL-PCR, T4 RNA ligase 2 specifically ligates a stem-loop adapter to mature tRNAs but not to precursor tRNAs or tRNA fragments. Subsequent TaqMan qRT-PCR amplifies only unmodified regions of the tRNA-adapter ligation products; therefore, FL-PCR quantification is not influenced by tRNA post-transcriptional modifications. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.
提供机构：
Taylor & Francis
创建时间：
2016-01-19
