Data from: Identification of robust yield QTL derived from cultivated emmer for durum wheat improvement

Phenotypic Data
Two durum × cultivated emmer recombinant inbred line (RIL) populations were evaluated for grain yield components under field conditions in North Dakota, USA. The BP025 population was developed by crossing Ben (PI 596557), a North Dakota hard amber durum variety, with PI 41025, a cultivated emmer accession collected near Samara, Russia. The BP025 population consists of 200 RILs developed by single seed-descent and was advanced to the F7:8 generation. The RP883 population was developed by crossing the durum line Rusty (PI 639869) with PI 193883, a cultivated emmer wheat accession collected near Shewa, Ethiopia. The RP883 population consists of 190 RILs developed by single seed-descent and was advanced to the F7:8 generation.
The two populations were evaluated under field conditions in a total of three seasons for each and were grown in a randomized complete block design (RCBD) with three replicates per season. Plants were grown in hill plots with each plot consisting of 10-15 seeds and considered an experimental unit. The BP025 population was planted and evaluated in 2017, 2018, and 2019. The RP883 population was evaluated in 2018, 2019, and 2020. The 2017, 2018, and 2019 plots were grown at the North Dakota State University field site near Prosper, ND (47.002°N, 97.115°W), and the 2020 plots were grown at the NDSU agronomy seed farm near Casselton, ND (46.880°N, 97.243°W). The BP025 and RP883 populations and parental lines were evaluated for 11 traits including days to heading (DTH), plant height (PHT), the total number of spikelets per spike (SPS), kernels per spike (KPS), grain weight per spike (GWS), thousand kernel weight (TKW), kernel area (KA), kernel width (KW), kernel length (KL), kernel circularity (KC), and kernel length:width ratio (KLW). DTH was measured as the number of days from planting until 50 % of the spikes emerged completely beyond the flag leaf. PHT was measured in centimeters from the base of the plot to the tip of the tallest spike (excluding awns). Eight heads were used for phenotypic evaluations. SPS was counted as the total number of spikelets per spike. KPS, GWS, TKW, KA, KW, KL, KC, and KLW were obtained using a MARVIN grain analyzer (GAT Sensorik GMBH, Neubrandenburg, Germany). For KPS and GWS, the value obtained by the MARVIN for each sample was divided by the number of spikes in that sample to obtain KPS and GWS for data analysis.
Genotypic Data
DNA of the BP025 population was extracted and genotyped using the Illumina iSelect 9k wheat SNP array. The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from PI 41025, “B” represents an allele from Ben, and “-“ indicates missing data. The data was used to assemble the linkage-based genetic maps for the 14 durum wheat chromosomes and further used in statistical analysis to identify chromosome regions harboring genes associated with the various phenotypic traits mentioned in the phenotypic file.
DNA of the RP883 population was extracted and genotyped using the Illumina iSelect 90k wheat SNP array. The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from Rusty, “B” represents an allele from PI 193883, and “-“ indicates missing data. The data was used to assemble the linkage-based genetic maps for the 14 durum wheat chromosomes and further used in statistical analysis to identify chromosome regions harboring genes associated with the various phenotypic traits mentioned in the phenotypic file.
Resources in this dataset:

Resource Title: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population BP025
File Name: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population BP025.xlsx
Resource Description: The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from PI 41025, “B” represents an allele from Ben, and “-“ indicates missing data.

Resource Title: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population RP883
File Name: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population RP883.xlsx
Resource Description: The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from Rusty, “B” represents an allele from PI 193883, and “-“ indicates missing data.

Resource Title: Phenotypic data collected from the durum x cultivated emmer wheat recombinant inbred populations BP025 and RP883
File Name: Phenotypic data collected from the durum x cultivated emmer wheat recombinant inbred populations BP025 and RP883.xlsx
Resource Description: In the data file, column headings indicate the trait evaluated, the year, and replicate or average of all three replicates. For example, “SPS2017rep1” indicates rep 1 of the spikelets per spike trait collected in the 2017 trial. Sheet 1 consists of the BP025 population data, and sheet 2 consists of the RP883 population data. An entry of ‘NA’ indicates missing data.

表型数据
两种 durum 与栽培二粒小麦重组自交系（RIL）群体在美国北达科他州的自然条件下进行了籽粒产量成分的评估。BP025 群体是通过将北达科他州硬 amber durum 品种 Ben（PI 596557）与在俄罗斯萨马拉附近收集的栽培二粒小麦品种 PI 41025 进行杂交而开发的。BP025 群体由 200 个通过单株种子繁殖的 RIL 组成，并进展到 F7:8 代。RP883 群体是通过将 durum 线 Rusty（PI 639869）与在埃塞俄比亚谢瓦附近收集的栽培二粒小麦品种 PI 193883 进行杂交而开发的。RP883 群体由 190 个通过单株种子繁殖的 RIL 组成，并进展到 F7:8 代。
这两个群体在每个群体总共三个季节的自然条件下进行了评估，并在随机完全区组设计（RCBD）中种植，每个季节有三个重复。植株在丘块中种植，每个丘块包含 10-15 粒种子，被视为一个实验单位。BP025 群体于 2017 年、2018 年和 2019 年种植和评估。RP883 群体于 2018 年、2019 年和 2020 年进行评估。2017 年、2018 年和 2019 年的丘块种植在北达科他州立大学位于北达科他州普罗瑟斯附近的试验场（47.002°N, 97.115°W），而 2020 年的丘块种植在北达科他州立大学农业种子农场附近的卡斯尔顿（46.880°N, 97.243°W）。BP025 和 RP883 群体及其亲本系在 11 个性状方面进行了评估，包括抽穗天数（DTH）、植株高度（PHT）、每穗总小穗数（SPS）、每穗籽粒数（KPS）、每穗籽粒重量（GWS）、千粒重（TKW）、籽粒面积（KA）、籽粒宽度（KW）、籽粒长度（KL）、籽粒圆形度（KC）和籽粒长度:宽度比（KLW）。DTH 的测量值为从播种到 50% 的穗完全从旗叶中伸出所需的天数。PHT 的测量值从丘块底部到最高穗尖（不包括芒）的厘米数。用于表型评估的穗数为八个。SPS 被计数为每穗的小穗总数。KPS、GWS、TKW、KA、KW、KL、KC 和 KLW 使用 MARVIN（GAT Sensorik GMBH，纽布兰登堡，德国）籽粒分析仪获得。对于 KPS 和 GWS，MARVIN 对每个样本的测量值除以该样本中的穗数，以获得用于数据分析的 KPS 和 GWS。
基因型数据
BP025 群体的 DNA 被提取并使用 Illumina iSelect 9k 小麦 SNP 阵列进行基因分型。基因型数据文件包含标记名称、标记的染色体分配、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用，其中“A”代表来自 PI 41025 的等位基因，“B”代表来自 Ben 的等位基因，“-”表示缺失数据。数据被用于组装基于连锁的遗传图谱，涉及 14 个 durum 小麦染色体，并进一步用于统计分析，以识别携带与表型文件中提到的各种表型性状相关的基因的染色体区域。
RP883 群体的 DNA 被提取并使用 Illumina iSelect 90k 小麦 SNP 阵列进行基因分型。基因型数据文件包含标记名称、标记的染色体分配、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用，其中“A”代表来自 Rusty 的等位基因，“B”代表来自 PI 193883 的等位基因，“-”表示缺失数据。数据被用于组装基于连锁的遗传图谱，涉及 14 个 durum 小麦染色体，并进一步用于统计分析，以识别携带与表型文件中提到的各种表型性状相关的基因的染色体区域。
本数据集中的资源：
资源标题：durum 与栽培二粒小麦重组自交系 BP025 的基因型数据
文件名：durum 与栽培二粒小麦重组自交系 BP025 的基因型数据.xlsx
资源描述：基因型数据文件包含标记名称、标记的染色体分配、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用，其中“A”代表来自 PI 41025 的等位基因，“B”代表来自 Ben 的等位基因，“-”表示缺失数据。
资源标题：durum 与栽培二粒小麦重组自交系 RP883 的基因型数据
文件名：durum 与栽培二粒小麦重组自交系 RP883 的基因型数据.xlsx
资源描述：基因型数据文件包含标记名称、标记的染色体分配、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用，其中“A”代表来自 Rusty 的等位基因，“B”代表来自 PI 193883 的等位基因，“-”表示缺失数据。
资源标题：从 durum 与栽培二粒小麦重组自交系 BP025 和 RP883 收集的表型数据
文件名：从 durum 与栽培二粒小麦重组自交系 BP025 和 RP883 收集的表型数据.xlsx
资源描述：在数据文件中，列标题表示评估的性状、年份和重复或所有三个重复的平均值。例如，“SPS2017rep1”表示在 2017 年试验中收集的每穗小穗数性状的 rep 1。工作表 1 包含 BP025 群体的数据，工作表 2 包含 RP883 群体的数据。‘NA’条目表示缺失数据。