Tcf-1 and HEB Cooperate to Establish the Epigenetic and Transcription Profile of CD4+CD8+ Thymocytes

Ablating either Tcf-1 or HEB in CD4+CD8+-thymocytes elicits similar developmental outcomes, including increased proliferation, decreased survival and reduced late Tcra rearrangements. We investigate how Tcf-1 and HEB co-regulate CD4+CD8+ thymocyte development by promoting chromatin accessibility, regulating nucleosome occupancy, and regulating gene expression at shared DNA binding-sites genome-wide.