Quantitative Analysis of Erythroblast Transcriptomes in Control and NUP-17d Treatment Groups

NUP-17d (17d) is an effective inhibitor of Plek2 protein. NUP-17d treatment inhibits erythroblast proliferation, differentiation and enucleation in a dose-dependent manner via directly binding to Plek2 protein. To reveal the detailed involving mechanism of NUP-17d treatment, we performed RNA sequencing to analyze the gene expression profiles change in erythroblasts which were treated with NUP-17d or DMSO (NC) for 48 hours in the EPO induction culture system. mRNA profiles of erythroblasts treated with NUP-17d or DMSO (NC) for 48 hours in the EPO induction culture system were generated by deep sequencing, in triplicate, using DNBseq SE50 RNA-Seq.