Transcript levels of genes in the wild type CAI4 as well as the gdt1/gdt1,the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutants. Candida albicans

We report the expression levels of transcripts of 5,901 genes in log-phase growing cells of the wild type CAI4 as well as the gdt1/gdt1, the pmr1/pmr1, and the gdt1/gdt1 pmr1/pmr1 mutants.We find that as compared to the wild type, there are 249, 446 and 440 differentially expressed genes (DEGs) in the gdt1/gdt1, the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutants, respectively. Deletion of CaGDT1 does not affect the transcript level of CaPMR1, and vice versa. There are 105 shared DEGs between the gdt1/gdt1 and the pmr1/pmr1 mutants, which accounts for 42% (105/249) of total DEGs due to deletion of CaGDT1 or 24% (105/446) of total DEGs due to deletion of CaPMR1. Overall design: RNA sequencing of four strains, the wild type CAI4, the gdt1/gdt1, the pmr1/pmr1, and the gdt1/gdt1 pmr1/pmr1 mutants, which carried the pCR4 vector with a URA3 marker and were grown to log phase in SD-URA medium at 30oC.