RNA Polymerase II transcription independent of TBP [NET-seq]

Using NET-seq to profile nascent RNA transcription, we show that auxin-mediated depletion of TBP does not affect Pol II transcription and gene activation via heat shock. Overall design: NET-seq analysis was performed in mouse embryonic stem cells, and is comprised of two biological replicates of untreated cells, auxin treated cells, heat shocked cells, and heat shocked + auxin treated cells (8 samples total).