Peripheral anatomy and central connectivity of proprioceptive sensory neurons in the Drosophila wing

Recent advances in electron microscopy (EM) and automated image segmentation have produced synaptic wiring diagrams of the Drosophila central nervous system. A limitation of existing fly connectome datasets is that most sensory neurons are excised during sample preparation, creating a gap between the central and peripheral nervous systems. Here, we bridge this gap by reconstructing wing sensory axons from the Female Adult Nerve Cord (FANC) EM dataset and mapping them to peripheral sensory structures using genetic tools and light microscopy. We confirm the location and identity of known wing mechanosensory neurons and identify previously uncharacterized axons, including a novel population of putative proprioceptors that make monosynaptic connections onto wing steering motor neurons. We also find that proprioceptors of adjacent campaniform sensilla on the wing have distinct axon morphologies and postsynaptic partners, suggesting a high degree of specialization in axon pathfinding and syna..., , # Peripheral anatomy and central connectivity of proprioceptive sensory neurons in the Drosophila wing

Dataset DOI: [10.5061/dryad.mgqnk99b5](10.5061/dryad.mgqnk99b5)

## Description of the data and file structure

We identified driver lines with sparse expression in the anterior dorsal mesothoracic wing nerve (ADMN) from FlyLight, a public resource showing driver line expression patterns in the central nervous system ([https://www.janelia.org/project-team/flylight](https://www.janelia.org/project-team/flylight)). We then ordered these driver lines from the Bloomington *Drosophila* Stock Center (BDSC), crossed them to a fluorescent reporter effector line (UAS-mCD8::GFP, or, for a few drivers, UAS-RedStinger;LexAopGFP.nls;ChAT-LexA to co-label with ChAT), and imaged the wing and wing hinge of the resulting progeny. For most images, native fluorescence was imaged. For images labelling actin, immunohistochemistry was used. See the Methods section of the accompanying manuscript ([https://d...,