DataSheet_1_Clinicopathologic and Genomic Features in Triple-Negative Breast Cancer Between Special and No-Special Morphologic Pattern.docx

BackgroundTriple-negative breast cancer (TNBC) is refractory and heterogeneous, comprising various entities with divergent phenotype, biology, and clinical presentation. As an aggressive subtype, Chinese TNBC patients with special morphologic patterns (STs) were restricted to its incidence of 10-15% in total TNBC population.MethodsWe recruited 89 patients with TNBC at Guangdong Provincial People’s Hospital (GDPH) from October 2014 to May 2021, comprising 72 cases of invasive ductal carcinoma of no-special type (NSTs) and 17 cases of STs. The clinical data of these patients was collected and statistically analyzed. Formalin-fixed, paraffin-embedded (FFPE) tumor tissues and matched blood samples were collected for targeted next-generation sequencing (NGS) with cancer-related, 520- or 33-gene assay. Immunohistochemical analysis of FFPE tissue sections was performed using anti-programmed cell death-ligand 1(PD-L1) and anti-androgen receptor antibodies.ResultsCases with NSTs presented with higher histologic grade and Ki-67 index rate than ST patients (NSTs to STs: grade I/II/III 1.4%, 16.7%,81.9% vs 0%, 29.4%, 58.8%; p

背景：三阴性乳腺癌（TNBC）具有抵抗性和异质性，包括具有不同表型、生物学特性和临床表现的多种实体。作为中国TNBC患者中具有特殊形态学模式（STs）的侵袭性亚型，其发病率仅限于总TNBC人群的10-15%。方法：本研究自2014年10月至2021年5月在广东省人民医院（GDPH）招募了89例TNBC患者，其中72例为非特殊型导管癌（NSTs），17例为STs。收集并统计分析了这些患者的临床数据。收集了石蜡包埋、福尔马林固定的（FFPE）肿瘤组织和匹配的血液样本，用于进行针对癌症相关基因的520或33基因靶向下一代测序（NGS）。采用抗程序性细胞死亡配体1（PD-L1）和抗雄激素受体抗体对FFPE组织切片进行免疫组化分析。结果：与STs患者相比，NSTs患者的组织学等级和Ki-67指数率更高（NSTs对STs：等级I/II/III 1.4%，16.7%，81.9% vs 0%，29.4%，58.8%；p