Histone linked genome in budding yeast Saccharomyces cerevisiae depending on growth conditions

S. cerevisiae CEN.PK 113-7A WT strain and it's ade4, ade8 , ade2 and ade16/17 were cultivated in SD media , then OD600 was adjusted to 0.5 and media were replaced with SD w/o nitrogen source, SD w/o purine source (for purine synthesis mutants). The study was supported by the Latvian Science Council's Fundamental and Applied Research project No. LZP-2021/1-0522 "From purine depletion to resilient phenotype - dissecting the mechanisms”. Samples prepared using CUTANA CUT&RUN Library Prep Kit (EpiCypher). Method adapted from Qasim MN, Valle Arevalo A, Paropkari AD, et al. Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow. J Vis Exp. 2022;(182):10.3791/63655. Published 2022 Apr 1. doi:10.3791/63655