Top 20 DnaA binding regions in vitro.

1The two binding regions in oriC are listed first, and subsequent peaks are ordered by the amounts of DNA recovered at 1.4 μM ATP-DnaA-his. For peaks in intergenic regions, both flanking genes are indicated. For peaks located within genes, only that gene is indicated.2The numbers presented are the genome coordinates for the summit of each peak. The genome sequence used was from lab strain AG1839 [29] with 1 being the nucleotide 409 bp upstream of the dnaA open reading frame.3The number of putative DnaA binding sites (DnaA boxes) in a 300 bp window centered on the peak summit, as determined using the PSSM (Materials and Methods).4Apparent Kd’s were determined as described (Materials and Methods) and are indicated for ATP-DnaA-his (ATP) and ADP-DnaA-his (ADP).Top 20 DnaA binding regions in vitro.