Combinatorial Immunotherapy Induces Tumor Infiltrating CD8+ T Cells with Distinct Functional, Migratory, and Stem-Like Properties III

This study reveals supeior efficiacy of triple combination treatment (TCT) based on anti-PD-(L)1 and anti-4-1BB/OX40 and describes immunological mechanisms underlying synergism between the treatment components Overall design: Using bulk RNA seq of TILs isolated from isotype- and TCT-treated mice sorted based on expression of PD-1 and KLRG1 into 4 distinct populations, we established a gene expression profile of key CD8+ T cell populations induced by the TCT treatment. Using scRNA-Seq of TILs isolated from isotype- and TCT-tretaed mice we defined a sunctional, phenotypic and developmental T cell heterogeneity within treated and non treated tumors.