Ole gene expression among tung tissues.

The qPCR reaction mixtures contained 5 ng of RNA-equivalent cDNA from various stages of tung tree 1 seeds, leaves and flowers, the optimized concentrations of each primer and probe (200 nM) and QPCR Mix. The expression levels under “seed” represent the means of the expression fold of 4 stages of seeds (weeks 2, 4, 6 and 10) (TaqMan qPCR) or 6 stages of seeds (weeks 2, 4, 5, 6, 8 and 10) (SYBR Green qPCR) calculated using three reference mRNA (Rpl19b, Gapdh and Ubl) with 2–4 assays for each stage. The expression levels under “leaf” and “flower” represent the means and standard deviations of the expression fold calculated using three reference mRNA (Rpl19b, Gapdh and Ubl) each with 2–4 assays and Ole gene expression in seeds as the calibrator. The mean CT values generated using RNA from leaves and flowers are included after expression fold as a proof of low but reliable levels of Ole mRNA detection in these tissues.