Host-pathogen transcriptomics of Mucor circinelloides NCRIP deficient spores phagocytosed by mouse macrophages

Here in this work, we uncovered the role of NCRIP in regulating virulent processes and transposable movements through key components of the pathway, RdRP1 and R3B2. Mutants in these genes are unable to launch a proper virulent response to macrophage phagocytosis, resulting in a decreased virulence potential. The transcriptomic profile of rdrp1? and r3b2? mutants revealed a pre-exposure adaptation to the stressful phagosomal environment even when the strains are not confronted by macrophages. Overall design: 8 samples are provided and analyzed, comprising 2 replicates (blank and R) for each coculture interacting samples and each single culture, which are as follows: Mucor r3b2 mutant strain cocultured with mouse macrophages (cell line J774A.1) for 5h, Mucor rdrp1 mutant strain co-cultured with mouse macrophages (cell line J774A.1) for 5h, Mucor r3b2 mutant strain (R7B) single-cultured in cell media for 5h, and Mucor rdrp1 mutant strain singlecultured in cell media for 5h. Libraries were prepared with Illumina TruSeq strand-specific mRNA kits and sequenced with an Illumina HiSeq 2500 system to generate 50-bp, first-strand (or reverse) and single-end reads.