Genome-wide mapping of H3K79me2 in EPZ5676-treated lymphatic endothelial cells [RNA-Seq]

Proper functioning of the lymphatic system is required for normal immune response, fluid balance and lipid reabsorption. Multiple regulatory mechanisms are employed to ensure correct formation of lymphatic vessels; however, whether epigenetic regulation is involved in this process remains largely unknown. We report that epigenetic priming by the Disruptor of telomeric silencing 1-like (DOT1L) in endothelial cell (EC) is indispensable for generation and function of lymphatic endothelial cells (LECs). Mechanistically, loss function of DOT1L leads to reduction of H3K79 di-methylation and expression of the genes important for LEC development and function. Thus, our study establishes DOT1L-mediated transcriptional regulation in ECs plays an important role in differentiation and function of LECs. Overall design: We perform RNA-Seq experiment in mouse E15.5 skin LECs (control vs Dot1l KO).