UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [shRNA]

Using pooled shRNA libraries we performed a focused screen of 115 genes in 3D ex vivo cultures of primary murine lung tumor cells and 2D cultures of murine LKR10 cells. These screens identify Uhrf1 as gene selectively important for the 3D growth of primary lung cancer spheroids. We analyze shRNA abundance at two different timepoints in primary tumor cells grown in 3D ex vivo cultures (5 sample pools x 6 replicates) and compare these results to a murine NSCLC cell line (LKR10) grown in 2D culture (5 sample pools x 6 replicates). The analysis compares T1 vs T0 groups.