Microarray analysis of Tm6sf2 E167K knockin mouse liver

The rs58542926 C>T variant of the transmembrane 6 superfamily member 2 gene (TM6SF2), encoding an E167K amino acid substitution, is associated with liver disease and cardiovascular disease. Therefore, we determined the long-term effects of this coding variant of TM6SF2 on glucose metabolism in mice with CRISPR/Cas9-mediated knock-in of TM6SF2 E167K (Tm6sf2 KI) and compared to littermate wild-type mice. To elucidate the molecular mechanisms that mediate the effects of the Tm6sf2 E167K variant on glucose and lipid metabolism, we performed microarray analysis in liver samples from the Tm6sf2 KI and Wt male mice fed a a high fat-diet (HFD) for 20 weeks.