Table_5_Quantitative Ubiquitylomic Analysis of the Dynamic Changes and Extensive Modulation of Ubiquitylation in Papaya During the Fruit Ripening Process.XLSX

Lysine ubiquitination is a highly conserved post-translational modification with diverse biological functions. However, there is little available information on lysine ubiquitination of non-histone proteins in papaya (Carica papaya L.). In total, 3,090 ubiquitination sites on 1,249 proteins with diverse localizations and functions were identified. Five conserved ubiquitinated K motifs were identified. Enrichment analysis showed that many Hsps were differentially ubiquitinated proteins (DUPs), suggesting an essential role of ubiquitination in degradation of molecular chaperone. Furthermore, 12 sugar metabolism-related enzymes were identified as DUPs, including an involvement of ubiquitination in nutrimental changes during the papaya ripening process. The ubiquitination levels of five fruit ripening-related DUPs, including one ethylene-inducible protein, two 1-aminocyclopropane-1-carboxylic acid oxidases, one endochitinase, and one cell wall invertase, were significantly changed during the ripening process. Our study extends the understanding of diverse functions for lysine ubiquitination in regulation of the papaya fruit ripening process.

赖氨酸泛素化是一种高度保守的翻译后修饰，具有多样的生物学功能。然而，关于木瓜（Carica papaya L.）非组蛋白赖氨酸泛素化的信息相对匮乏。本研究共鉴定出1,249种蛋白上的3,090个泛素化位点，这些位点具有不同的定位和功能。发现了五种保守的泛素化K结构域。富集分析表明，许多热休克蛋白（Hsps）是差异泛素化蛋白（DUPs），暗示泛素化在分子伴侣降解过程中发挥着至关重要的作用。此外，还鉴定出12种与糖代谢相关的酶为DUPs，其中包括泛素化在木瓜成熟过程中营养变化中的参与。在木瓜成熟过程中，包括一个乙烯诱导蛋白、两个1-氨基环丙烷-1-羧酸氧化酶、一个内切几丁质酶和一个细胞壁 invertase在内的五种与果实成熟相关的DUPs的泛素化水平发生了显著变化。本研究拓展了赖氨酸泛素化在调节木瓜果实成熟过程中的多种功能理解。