FOXO-regulated Deaf1 controls muscle regeneration through autophagy

We performed RNAi screens to identify regulators of muscle regeneration. We identified Deaf1 and hypothesized that this transcription factor may regulate autophagy and in turn control myogenesis. We therefore performed chromatin-immunoprecipitation followed by next-generation sequencing (ChIP-seq) to identify Deaf1 downstream targets. The results suggest that Deaf1 binds to the promoters the multiple ATG genes, suppressing their transcription. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Deaf1 in C2C12 mouse myoblast cells.