SRC2 and SRC3 cooperatively promote pathogenic function of Th17 cells via regulation of epigenetic activation

Steroid receptor coactivator 3 (SRC3) was reported to regulates pathogenic Th17 cell differentiation in vitro and in vivo.However, the role of SRC2, another member of SRC family, and its relationship with SRC3 in the regulation of Th17 cell differentiation remain unknown. We used RNA-seq to study the global function of SRC2 and SRC3. Naive CD4+ T cells were activated with 5ug/ml plate-bound anti-CD3/28, and cultured with TGF-β+IL-6 or with IL-6+IL-1β+IL23. 4days later, cells were restimulated with plate-bound anti-CD3 for 4hrs for RNA-seq. To understand the overall function of SRC2 and SRC3 in Th17 cells, RNA-seq was performed with WT, Src2 KO and Src2/3 DKO in in vitro cultured Th17 cells.