*p-value for mean total RNA concentration between methods. Total RNA concentration and purity for Trizol LS and Qiagen miRNeasy with RNA yeast carrier.
P-values generated from pairwise t-tests comparing the percentage of Twist target probes covered by at least one read for each capture efficiency experiment. 65C/68C refers to annealing temperature an
aThe time for centrifugation/vortexing, incubation steps and hands-on time was determined. bDuring centrifugation, time was used for preparation of next steps including labeling. Thus, total duration
BACKGROUND: SAGE (serial analysis of gene expression) is a recently developed technique for systematic analysis of eukaryotic transcriptomes. The most critical step in the SAGE method is large scale a
