Analysis of nonsense-mediated mRNA decay in daf-2 mutants

To exmine the role of nonsense-mediated mRNA decay process in the longevity regulation of daf-2 mutants, we sequenced transcriptomes from day 1 adult Caenorhabditis elegans: Bristol N2 (wild-type), and smg-2(qd101), daf-2(e1370) and smg-2(qd101); daf-2(e1370) mutants. Determination of transcript levels in N2 (wild-type), smg-2(qd101), daf-2(e1370), and smg-2(qd101); daf-2(e1370) animals by using deep sequencing, in duplicate