3D ED/MicroED of the class Ia ribonucleotide reductase R2 subunit in oxidized FeIII-FeIII form (metR2a)

This upload includes the individual converted datasets (IMG format) and XDS output files from data reduction. Datasets were originally recorded as MRC files and converted to IMG format using an in-house script. Datasets were processed with XDS and merged using edtools. The final merged dataset included these 22 crystals with data to 2.00 Å resolution, 88.1% completeness, CC1/2 of 98.4%, and I/σ(I) of 5.73. All MicroED datasets were collected on a Titan Krios cryo-TEM (ThermoFisher Scientific) operating at 300 kV with a Ceta-D CMOS detector. The software EPU-D was used for MicroED data collection. Dataset Tilt step [°/frame] Exposure time [s] No. frames [-] Tilt range [°] Flux [eÅ-2s-1] Fluence [eÅ-2] C2 [µm] CL [m] Beam Size [µm] Spot size [-] 1 0.5 0.5 129 64.5 0.21 13.3 50 1.5 3 8 2 0.5 1 199 99.5 0.01 2.0 20 1.2 2.5 8 3 0.5 0.5 199 99.5 0.01 1.0 20 1.2 2.5 8 4 0.5 0.5 199 99.5 0.01 1.0 20 1.2 2.5 8 5 0.5 1 199 99.5 0.01 2.0 20 1.2 2.5 8 6 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 7 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 8 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 9 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 10 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 11 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 12 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 13 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 14 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 15 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 16 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 17 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 18 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 19 0.2 0.5 99 19.8 0.21 10.2 50 1.5 3 8 20 0.2 0.5 99 19.8 0.05 2.6 50 1.5 3 10 21 0.5 0.5 159 79.5 0.21 16.4 50 1.5 3 8 22 0.5 0.5 159 79.5 0.21 16.4 50 1.5 3 8