RNA-seq to identify P-body associated mRNAs upon glucose starvation, Na+ and Ca2+ stresses

To examine mRNA species sequestered by P-bodies upon different stress conditions, we employed a modified crosslinking immunoprecipitation (cCLAP) technique to purify P-body associated mRNAs and analyzed by RNA-Seq. To compare, we also performed total RNA-Seq under the same stress conditions.为探究不同应激条件下被加工小体（P-bodies）所封存的信使核糖核酸（mRNA）种类，我们采用改良交联免疫沉淀（cCLAP）技术纯化与加工小体结合的mRNA，并通过RNA测序（RNA-Seq）对其进行分析。为进行对照比较，我们还在相同应激条件下开展了总RNA测序（total RNA-Seq）实验。