Effect of peripheral (IP) and central (intra-VTA) WIN 55,212-2 and IP AM251 on c-Fos IR in A^y and WT mice.

Male Ay and WT mice were perfused with 4% paraformaldehyde 90 min after IP injection with saline-DMSO (n = 3), WIN 55,212-2 (1 mg/kg;n = 3) or AM251 (5 mg/kg; n = 4). Another group of male Ay and WT mice was perfused 90 min after intra-VTA injection with saline-DMSO (n = 3), WIN 55,212-2 (1 µg; n = 3) or AM251 (1 µg; n = 4). Immunohistochemical staining for c-Fos expression in nucleus accumbens, NAc (shell), arcuate nucleus (ARH), paraventricular nucleus (PVH), lateral hypothalamus (LH), dorsomedial hypothalamus (DMH), ventromedial hypothalamus (VMH), central amygdala (CeA), ventral tegmental area (VTA), locus coerulus (LC), parabrachial nucleus (PBN) and nucleus of the solitary tract (NTS). Results are expressed as mean±SEM.* p <0.05, ** p <0.01, *** p <0.001 compared to saline; † represent statistical comparisons between WT and Ay mice; † p <0.05,†† p <0.01, ††† p <0.001.