Babesia Infected/Control LongSAGE libraries for Boophilus microplus ovary tissue
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE9880
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Babesia infected (and control) ovary tissue was used to construct LongSAGE libraries. The intent was to find differential gene expression in both libraries. Details: The extraction was done using Ambion's ToTALLY RNA kit, and the concatemers where constructed using Invitrogen's I-SAGE Long kit. The concatmer files (multiple fasta sequence files) were processed using in-house perl scripts to extract the 17bp LongSAGE tags. **Note: contact person: Felix D. Guerrero. email: felix.guerrero@ars.usda.gov Keywords: LongSAGE 1 control and 1 treatment library was constructed. Besides the ovary, gut was also looked at but the data was un-analyzable. ************** Analysis Results ********** Ovary Infected: Project tags: 8750 Project files(concatemers/sequences): 703 Project Duplicate Dimers(ditags): 1612 Unique tags 5315 Total tags inc. ditags 11974 % of tags in duplicate ditags 36% Ovary Control: Project tags: 6764 Project files(concatemers/sequences): 368 Project Duplicate Dimers(ditags): 846 Unique tags 3937 Total tags inc. ditags 8456 % of tags in duplicate ditags 25%
创建时间：
2012-03-17
