GNPS Outdoor versus indoor cultivation: effects on the metabolite profile of Agaricus subrufescens strains analyzed by untargeted metabolomics

Five commercial strains of the culinary and medicinal mushroom Agaricus subrufescens were grown under two cultivation systems (outdoor versus indoor). Polar and lipid extracts of mushroom dry powders were extracted with a mixture of solvents (water:methanol:methyl tert-buthyl ether) and analyzed by UHPLC-ESI-HRMS-based untargeted metabolomics. A Nexera X2 UHPLC system (Shimadzu) equipped with an Acquity UPLC HSS T3 column (2.1 mm x 100 mm x 1.8 um) (Waters) was employed coupled to a MaXis 4G Q-TOF MS analyzer (Bruker Daltonics) through an electrospray ionization source. Data was acquired in positive ion mode.