TABLE 2 in How complex is the Naineris setosa species complex? First integrative study of a presumed cosmopolitan and invasive annelid (Sedentaria: Orbiniidae)

TABLE 2. Primers and PCR protocols used in this study.
MarkerCycle (PCR)Primer nameSequence (5’–3’)DirectionReference1x5x1xCOI94°C - 40 s94°C - 40 s45°C - 40 s72°C - 120 s72°C - 7 minpolyLCOGAYTATWTTCAACAAATCATAAAGATATTGGForwardCarr et al. (2011)40x94°C - 40 s48°C - 40 s72°C - 120 spolyHCOTAMACTTCWGGGTGACCAAARAATCAReverseCarr et al. (2011)1x40x1x16S94°C - 3 min94°C - 30 s51°C - 30 s (-0.2C per cycle)72°C - 70 s72°C - 7 min16SarLCGCCTGTTTATCAAAAACATForwardPalumbi et al. (1991)16SbrHCCGGTCTGAACTCAGATCACGTReversePalumbi et al. (1991)1x40x1x16S94°C - 3 min94°C - 30 s52°C - 30 s72°C - 70 s72°C - 7 minAnnFGCGGTATCCTGACCGTRCWAAGGTAForwardSjölin et al. (2005)AnnRTCCTAAGCCAACATCGAGGTGCCAAReverseSjölin et al. (2005)1x40x1x28S94°C - 40 s94°C - 40 s54°C - 40 s72°C - 120 s72°C - 7 min28v (28gg)AAGGTAGCCAAATGCCTCGTCATCForwardHillis & Dixon (1991)28x (28ii)GTGAATTCTGCTTCACAATGATAGGAAGAGCCReverseHillis & Dixon (1991)