E2P4 COVID19 Trial Cytokine profile data

E2P4 treated COVID-19 donor serum samples, cytokine protein expression data set. Proteins were measured using the Olink® Target 48 Cytokine panel* (Olink Proteomics AB, Uppsala, Sweden) according to the manufacturer's instructions. The Proximity Extension Assay (PEA) technology used for the Olink protocol has been well described,49 and enables 45 analytes to be analyzed simultaneously, using 1 µL of each sample. In brief, pairs of oligonucleotide-labeled antibody probes bind to their targeted protein, and if the two probes are brought in close proximity the oligonucleotides will hybridize in a pair-wise manner. The addition of a DNA polymerase leads to a proximity-dependent DNA polymerization event, generating a unique PCR target sequence. The resulting DNA sequence is subsequently detected and quantified using a microfluidic real-time PCR instrument (Biomark HD, Fluidigm). Data is then quality controlled and normalized using an internal extension control and calibrators, to adjust for intra- and inter-run variation. The final assay read-out is presented in pg/ml using a 4-Pl fit for absolute quantification. All assay validation data (detection limits, intra- and inter-assay precision data, etc.) are available on manufacturer's website (www.olink.com).