Data for: Phylogenetic structure of body shape in a diverse inland ichthyofauna

Body shape is a fundamental metric of animal diversity affecting critical behavioral and ecological dynamics and conservation status, yet previously available methods capture only a fraction of total body-shape variance. Here we use structure-from-motion (SFM) 3D photogrammetry to generate digital 3D models of adult fishes from the Lower Mississippi Basin, one of the most diverse temperate-zone freshwater faunas on Earth, and 3D geometric morphometrics to capture morphologically distinct shape variables, interpreting principal components as growth fields. The mean body shape in this fauna resembles plesiomorphic teleost fishes, and the major dimensions of body-shape disparity are similar to those of other fish faunas worldwide. Major patterns of body-shape disparity are structured by phylogeny, with nested clades occupying distinct portions of the morphospace, most of the morphospace occupied by multiple distinct clades, and one clade (Acanthomorpha) accounting for over half of the total body shape variance. In contrast to previous studies, variance in body depth (59.4%) structures overall body-shape disparity more than does length (31.1%), while width accounts for a non-trivial (9.5%) amount of the total body-shape disparity.

Methods
3D models of fish specimens from the Lower Mississippi Basin were created using SFM 3D photogrammetry. The models were then landmarked in three dimensions using the free program Slicer. Landmark files for each model were exported from Slicer and are found in the "3D_landmarks" folder. The landmarks files were loaded back into Slicer and processed using the GPA module. The GPA module outputs were exported as the folder "Slicer_Output".