Analysis of EGFR signaling pathway; miRNAs and inflammatory biomarkers in a high-risk oral cancer population in Pakistan

Oral cancer has high morbidity rates in the Asian region, specifically Pakistan. However, little insight is available regarding the molecular pathogenesis and inflammatory biomarkers of this preventable cancer. This study determined the association of EGFR, NFκB, COX-2, and miRNAs expression with the chewing habits and high-risk human papillomavirus (HR-HPV) infection in oral cancer patients.**** Formalin-fixed embedded blocks (FFPPE) (n=50) were analyzed for transcriptional expression of EGFR, NFκB, and COX-2 by qPCR, and COX-2 protein expression was checked by immunohistochemistry (IHC). Array profiling of ~2500 miRNAs was performed on nine samples, and five miRNAs were selected for validation from this profiling data. Appropriate statistical tests were applied to check the association of EGFR., NFκB, COX-2, and miRNAs with chewing and HR-HPV status, (p<0.05 and 95% CI).**** Of the 50 samples, transcriptional expression of EGFR was observed in 13 (26%), NFκB in 11 (22%), and COX-2 ..., Formalin-fixed embedded blocks (FFPPE) (n=50) were analyzed for transcriptional expression of EGFR, NFκB, and COX-2 by qPCR and COX-2 protein expression was checked by immunohistochemistry (IHC). Array profiling of ~2500 miRNAs was performed on nine samples, and five miRNAs were selected for validation from this profiling data. Appropriate statistical tests were applied to check the association of EGFR., NFκB, COX-2, and miRNAs with chewing and HR-HPV status, (p<0.05 and 95% CI). , , # Analysis of EGFR signaling pathway; miRNAs and inflammatory biomarkers in a high-risk oral cancer population in Pakistan

[https://doi.org/10.5061/dryad.q573n5tsr](https://doi.org/10.5061/dryad.q573n5tsr)

## Description of the data and file structure

Formalin-fixed embedded oral squamous cell carcinoma blocks (FFPPE) (n=50) were analyzed for transcriptional expression of EGFR, NFκB, and COX-2 by qPCR and COX-2 protein expression was checked by immunohistochemistry (IHC). Array profiling of ~2500 miRNAs was performed on nine samples, and five miRNAs were selected for validation from this profiling data. Appropriate statistical tests were applied to check the association of EGFR., NFκB, COX-2, and miRNAs with chewing and HR-HPV status, (p<0.05 and 95% CI).

### Files and variables

#### File: Namra1(5.2.19)_for_analysis-working.sav

**Description:** 

The file has variables of chewing habits, EGFR performed by FISH, HPV status, EGFR, COX-2, NFKB transcriptional expression, Gender, Smo..., All the data was collected after ethical approval from Aga Khan University Hospital ERC. Consent was obtained from the subjects, and the data is published keeping complete anonymity. No direct or indirect identifier is used which could breach the anonymity of the data.