Replication data for: “Development of a three-dimensional vitrification protocol for domestic cat cumulus-oocyte complexes and comparison with standard vitrification"

This dataset contains replication data for the manuscript: “Development of a three-dimensional vitrification protocol for domestic cat cumulus-oocyte complexes and comparison with standard vitrification”.

Sodium alginate was employed as a matrix for the encapsulation of cat cumulus-oocyte complexes. Four experiments were performed to assess: 1) the optimal concentration of alginate; 2) the permeability of alginate to cryoprotectants used for vitrification; 3) the effect of vitrification in the alginate matrix on oocyte morpho-functionality and 4) the effect of vitrification in the alginate matrix on oocyte development into embryos in vitro.

Data of each experiment consists of:
1) viability, nuclear status and cumulus expansion of fresh cumulus-oocyte complexes encapsulated in alginate at different concentrations;
2) concentration of the cryoprotectants dimethyl sulfoxide (DMSO) and ethylene glycol (EG) in fresh cumulus-oocyte complexes after exposure in standard conditions or after encapsulation in sodium alginate; bovine cumulus-oocyte complexes were used as a model considering the availability of biological material;
3) viability, cytoskeletal integrity and nuclear status of cumulus-oocyte complexes following standard vitrification or vitrification in the alginate matrix;
4) embryo development of cumulus-oocyte complexes following standard vitrification or vitrification in the alginate matrix.

This work was supported under the National Recovery and Resilience Plan (“PNRR”), Mission 4, Component 2, Investment 1.1, Call for tender No. 1409 published on September 14, 2022 by the Italian Ministry of University and Research (MUR), funded by the European Union – NextGenerationEU – PRIN 2022 PNRR n. P2022PRFM7 – Project Title “Bioengineered 3D ovarian follicle for biodiversity preservation – Bio3versity” – CUP G53D23007780001 – Grant Assignment Decree No. 1377 adopted on September 01, 2023 by the Italian Ministry of Ministry of University and Research (MUR).