Tissue-based mapping of the fathead minnow (Pimephales promelas) transcriptome and proteome

Omics approaches are broadly used to explore endocrine and toxicity-related pathways and functions. Nevertheless, there is still a significant gap in knowledge in terms of understanding the endocrine system and its numerous connections and intricate feedback loops, especially in non-model organisms. The main purpose of this analysis was to generate tissue-specific omics data in order to support future aquatic ecotoxicogenomic and endocrine-related studies as well as to improve our understanding of the fathead minnow as an ecological model. All fish husbandry was conducted under the supervision of the University of Florida Institutional Animal Care and Use Committee. Adult fathead minnows (Pimephales promelas) were obtained from an in-house culture at the Aquatic Toxicology Core Laboratory at the University. The main objective of this study was to increase the value of the FHM as a model by creating comprehensive transcriptomic and proteomic databases. This study also aims to survey tissue-specific baseline transcriptomic and proteomic expression profiles in adult male FHM to support aquatic ecotoxicogenomic studies.